› 1isr

X-ray diffraction
4Å resolution

Crystal Structure of Metabotropic Glutamate Receptor Subtype 1 Complexed with Glutamate and Gadolinium Ion

Released:
DOI: 10.2210/pdb1isr/pdb
PDB entry 1isr coloured by chain, front view.
PDB entry 1isr coloured by chain, side view.
PDB entry 1isr coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural views of the ligand-binding cores of a metabotropic glutamate receptor complexed with an antagonist and both glutamate and Gd3+.
Tsuchiya D, Kunishima N, Kamiya N, Jingami H, Morikawa K
Proc Natl Acad Sci U S A 99 2660-5 (2002)
PMID: 11867751

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metabotropic glutamate receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 55.26 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23385 (Residues: 33-522; Coverage: 42%)
Gene names: Gprc1a, Grm1, Mglur1
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GLU 1 x GLU
Ligand GD 2 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P3221
Unit cell:
a: 145.348Å b: 145.348Å c: 76.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.237 0.259
Expression system: Spodoptera frugiperda

Quick links

Citations

70 review citations

Metabotropic glutamate receptors: physiology, pharmacology, and disease.
Niswender et al. (2010)
69 more

22 mentions without citation

Metabotropic glutamate receptors: from the workbench to the bedside.
Nicoletti et al. (2011)
21 more