› 1ike

X-ray diffraction
1.5Å resolution

Crystal Structure of Nitrophorin 4 from Rhodnius Prolixus Complexed with Histamine at 1.5 A Resolution

Released:
DOI: 10.2210/pdb1ike/pdb
PDB entry 1ike coloured by chain, front view.
PDB entry 1ike coloured by chain, side view.
PDB entry 1ike coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Ligand-induced heme ruffling and bent no geometry in ultra-high-resolution structures of nitrophorin 4.
Roberts SA, Weichsel A, Qiu Y, Shelnutt JA, Walker FA, Montfort WR
Biochemistry 40 11327-37 (2001)
PMID: 11560480

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand HSM 1 x HSM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2
Unit cell:
a: 70.18Å b: 42.52Å c: 52.86Å
α: 90° β: 94.35° γ: 90°
R-values:
R R work R free
0.18 0.18 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Hemoglobin and heme scavenging.
Ascenzi et al. (2005)
9 more

17 mentions without citation

The Immunobiology of the Interleukin-12 Family: Room for Discovery.
Tait Wojno et al. (2019)
16 more