› 1iho

X-ray diffraction
1.7Å resolution

CRYSTAL APO-STRUCTURE OF PANTOTHENATE SYNTHETASE FROM E. COLI

Released:
DOI: 10.2210/pdb1iho/pdb
PDB entry 1iho coloured by chain, front view.
PDB entry 1iho coloured by chain, side view.
PDB entry 1iho coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of E. coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily.
von Delft F, Lewendon A, Dhanaraj V, Blundell TL, Abell C, Smith AG
Structure 9 439-50 (2001)
PMID: 11377204

Function and Biology Details

Reaction catalysed: 
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pantothenate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31663 (Residues: 1-283; Coverage: 100%)
Gene names: JW0129, b0133, panC
Sequence domains: Pantoate-beta-alanine ligase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 66.031Å b: 78.075Å c: 77.126Å
α: 90° β: 103.71° γ: 90°
R-values:
R R work R free
0.211 0.212 0.254
Expression system: Escherichia coli

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Citations

7 review citations

Coenzyme A biosynthesis: an antimicrobial drug target.
Spry et al. (2008)
6 more

7 mentions without citation

Sequence co-evolution gives 3D contacts and structures of protein complexes.
Hopf et al. (2014)
6 more