› 1ibj

X-ray diffraction
2.3Å resolution

Crystal structure of cystathionine beta-lyase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb1ibj/pdb
PDB entry 1ibj coloured by chain, front view.
PDB entry 1ibj coloured by chain, side view.
PDB entry 1ibj coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.
Breitinger U, Clausen T, Ehlert S, Huber R, Laber B, Schmidt F, Pohl E, Messerschmidt A
Plant Physiol 126 631-42 (2001)
PMID: 11402193

Function and Biology Details

Reaction catalysed: 
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-lyase, chloroplastic Chains: A, C
Molecule details ›
Chains: A, C
Length: 464 amino acids
Theoretical weight: 50.49 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P53780 (Residues: 1-464; Coverage: 100%)
Gene names: At3g57050, F24I3.130
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand CO3 2 x CO3
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 107.75Å b: 154.332Å c: 118.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.249 0.309
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The aspartic acid metabolic pathway, an exciting and essential pathway in plants.
Azevedo et al. (2006)
4 more

5 mentions without citation

Crystal structure of Homo sapiens kynureninase.
Lima et al. (2007)
4 more