Function and Biology Details
Reactions catalysed:
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:
Structure analysis Details
Assemblies composition:
Assembly name:
Caspase-7 and E3 ubiquitin-protein ligase XIAP (preferred)
PDBe Complex ID:
PDB-CPX-157230 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-7
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 31.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Length: 280 amino acids
Theoretical weight: 31.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P55210 (Residues: 24-303; Coverage: 92%)
Sequence domains: Caspase domain
Structure domains: Rossmann fold
E3 ubiquitin-protein ligase XIAP
Molecule details ›
Chains: C, D
Length: 141 amino acids
Theoretical weight: 16.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Inhibitor of Apoptosis domain
Length: 141 amino acids
Theoretical weight: 16.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P98170 (Residues: 120-260; Coverage: 28%)
Sequence domains: Inhibitor of Apoptosis domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
CHESS BEAMLINE F1
Spacegroup:
P3221
Expression system: Escherichia coli
