› 1hxi

X-ray diffraction
1.6Å resolution

AN UNEXPECTED EXTENDED CONFORMATION FOR THE THIRD TPR MOTIF OF THE PEROXIN PEX5 FROM TRYPANOSOMA BRUCEI

Released:
DOI: 10.2210/pdb1hxi/pdb
PDB entry 1hxi coloured by chain, front view.
PDB entry 1hxi coloured by chain, side view.
PDB entry 1hxi coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
An unexpected extended conformation for the third TPR motif of the peroxin PEX5 from Trypanosoma brucei.
Kumar A, Roach C, Hirsh IS, Turley S, deWalque S, Michels PA, Hol WG
J Mol Biol 307 271-82 (2001)
PMID: 11243819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome targeting signal 1 receptor PEX5 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.71 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U7C3 (Residues: 333-453; Coverage: 19%)
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P65
Unit cell:
a: 75Å b: 75Å c: 56.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.186 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Versatile TPR domains accommodate different modes of target protein recognition and function.
Allan et al. (2011)
7 more

4 mentions without citation

TPRpred: a tool for prediction of TPR-, PPR- and SEL1-like repeats from protein sequences.
Karpenahalli et al. (2007)
3 more