› 1huy

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF CITRINE, AN IMPROVED YELLOW VARIANT OF GREEN FLUORESCENT PROTEIN

Released:
DOI: 10.2210/pdb1huy/pdb
PDB entry 1huy coloured by chain, front view.
PDB entry 1huy coloured by chain, side view.
PDB entry 1huy coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications.
Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY
J Biol Chem 276 29188-94 (2001)
PMID: 11387331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.32 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 52.504Å b: 61.758Å c: 70.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.208
Expression system: Escherichia coli

Quick links

Citations

152 review citations

A guide to choosing fluorescent proteins.
Shaner et al. (2005)
151 more

11 mentions without citation

Advances in imaging of understudied ions in signaling: A focus on magnesium.
Lazarou et al. (2020)
10 more