› 1hr8

X-ray diffraction
2.7Å resolution

Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Cytochrome C Oxidase IV Signal Peptide

Released:
DOI: 10.2210/pdb1hr8/pdb
PDB entry 1hr8 coloured by chain, front view.
PDB entry 1hr8 coloured by chain, side view.
PDB entry 1hr8 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences.
Taylor AB, Smith BS, Kitada S, Kojima K, Miyaura H, Otwinowski Z, Ito A, Deisenhofer J
Structure 9 615-25 (2001)
PMID: 11470436

Function and Biology Details

Reaction catalysed: 
Release of N-terminal targeting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-137328 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mitochondrial-processing peptidase subunit alpha Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 475 amino acids
Theoretical weight: 52.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11914 (Residues: 14-482; Coverage: 97%)
Gene names: MAS2, MIF2, YHR024C
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Mitochondrial-processing peptidase subunit beta Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 443 amino acids
Theoretical weight: 48.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10507 (Residues: 21-462; Coverage: 96%)
Gene names: L9632.10, MAS1, MIF1, YLR163C
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome c oxidase subunit 4, mitochondrial Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 24 amino acids
Theoretical weight: 2.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P04037 (Residues: 2-25; Coverage: 16%)
Gene names: COX4, G1362, YGL187C

Ligands and Environments

2 bound ligands:
Ligand EPE 2 x EPE
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 134.26Å b: 178.12Å c: 202.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

36 review citations

Importing mitochondrial proteins: machineries and mechanisms.
Chacinska et al. (2009)
35 more

5 mentions without citation

Mitochondrial Processing Peptidases-Structure, Function and the Role in Human Diseases.
Kunová et al. (2022)
4 more