› 1hkc

X-ray diffraction
2.8Å resolution

RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE

Released:
DOI: 10.2210/pdb1hkc/pdb
PDB entry 1hkc coloured by chain, front view.
PDB entry 1hkc coloured by chain, side view.
PDB entry 1hkc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate.
Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB
J Mol Biol 282 345-57 (1998)
PMID: 9735292

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-1 Chain: A
Molecule details ›
Chain: A
Length: 917 amino acids
Theoretical weight: 102.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19367 (Residues: 1-917; Coverage: 100%)
Gene name: HK1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BGC 1 x BGC
Ligand PO4 2 x PO4
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3221
Unit cell:
a: 175.09Å b: 175.09Å c: 99.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Molecular physiology of mammalian glucokinase.
Iynedjian PB. (2009)
2 more

11 mentions without citation

Deamidation of human proteins.
Robinson et al. (2001)
10 more