› 1hbx

X-ray diffraction
3.15Å resolution

Ternary Complex of SAP-1 and SRF with specific SRE DNA

Released:
DOI: 10.2210/pdb1hbx/pdb
PDB entry 1hbx coloured by chain, front view.
PDB entry 1hbx coloured by chain, side view.
PDB entry 1hbx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex.
Hassler M, Richmond TJ
EMBO J 20 3018-28 (2001)
PMID: 11406578

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113892 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Serum response factor Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11831 (Residues: 132-223; Coverage: 18%)
Gene name: SRF
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
ETS domain-containing protein Elk-4 Chains: G, H
Molecule details ›
Chains: G, H
Length: 157 amino acids
Theoretical weight: 17.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28324 (Residues: 2-156; Coverage: 36%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*AP*TP*GP*GP*CP*CP*TP*AP*AP*TP*TP*AP* GP*GP*AP*CP*TP*TP*CP*CP*GP*GP*TP*G)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
5'-D(*CP*AP*CP*AP*CP*CP*GP*GP*AP*AP*GP*TP*CP* CP*TP*AP*AP*TP*TP*AP*GP*GP*CP*CP*AP*T)-3' Chains: W, X
Molecule details ›
Chains: W, X
Length: 26 nucleotides
Theoretical weight: 7.94 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 142.68Å b: 144.39Å c: 75.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The ETS-domain transcription factor family.
Sharrocks AD. (2001)
16 more

11 mentions without citation

Sequence and structural analyses of nuclear export signals in the NESdb database.
Xu et al. (2012)
10 more