› 1haw

X-ray diffraction
1.9Å resolution

X-RAY STRUCTURE OF A BLUE COPPER NITRITE REDUCTASE AT HIGH PH AND IN COPPER FREE FORM AT 1.9 A RESOLUTION

Released:
DOI: 10.2210/pdb1haw/pdb
PDB entry 1haw coloured by chain, front view.
PDB entry 1haw coloured by chain, side view.
PDB entry 1haw coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
X-ray structure of a blue copper nitrite reductase at high pH and in copper-free form at 1.9 A resolution.
Ellis MJ, Dodd FE, Strange RW, Prudêncio M, Sawers G, Eady RR, Hasnain SS
Acta Crystallogr D Biol Crystallogr 57 1110-8 (2001)
PMID: 11468394

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.57 KDa
Source organism: Achromobacter xylosoxidans
UniProt:
  • Canonical: O68601 (Residues: 25-360; Coverage: 100%)
Gene names: O9570_16215, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P63
Unit cell:
a: 106.106Å b: 106.106Å c: 63.647Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.199

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Citations

1 review citation

Recent structural insights into the function of copper nitrite reductases.
Horrell et al. (2017)
12 other articles