› 1go8

X-ray diffraction
2Å resolution

The metzincin's methionine: PrtC M226L mutant

Released:
DOI: 10.2210/pdb1go8/pdb
PDB entry 1go8 coloured by chain, front view.
PDB entry 1go8 coloured by chain, side view.
PDB entry 1go8 coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond.
Hege T, Feltzer RE, Gray RD, Baumann U
J Biol Chem 276 35087-92 (2001)
PMID: 11445573

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of bonds with hydrophobic residues in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serralysin C Chain: P
Molecule details ›
Chain: P
Length: 462 amino acids
Theoretical weight: 49.75 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P16317 (Residues: 18-479; Coverage: 97%)
Gene name: prtC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand ZN 1 x ZN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 102.015Å b: 102.015Å c: 121.302Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.169 0.197
Expression system: Escherichia coli

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Citations

9 review citations

Structural aspects of the metzincin clan of metalloendopeptidases.
Gomis-Rüth FX. (2003)
8 more