› 1gn0

X-ray diffraction
1.8Å resolution

Escherichia coli GlpE sulfurtransferase soaked with KCN

Released:
DOI: 10.2210/pdb1gn0/pdb
PDB entry 1gn0 coloured by chain, front view.
PDB entry 1gn0 coloured by chain, side view.
PDB entry 1gn0 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Escherichia coli GlpE is a prototype sulfurtransferase for the single-domain rhodanese homology superfamily.
Spallarossa A, Donahue JL, Larson TJ, Bolognesi M, Bordo D
Structure 9 1117-25 (2001)
PMID: 11709175

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate sulfurtransferase GlpE Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.09 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6V5 (Residues: 1-108; Coverage: 100%)
Gene names: JW3388, b3425, glpE
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P32
Unit cell:
a: 53.796Å b: 53.796Å c: 30.346Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.173 0.248
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The rhodanese/Cdc25 phosphatase superfamily. Sequence-structure-function relations.
Bordo et al. (2002)
4 more

3 mentions without citation

Computational protein design quantifies structural constraints on amino acid covariation.
Ollikainen et al. (2013)
2 more