› 1gki

X-ray diffraction
3Å resolution

Plasmid coupling protein TrwB in complex with ADP and Mg2+.

Released:
DOI: 10.2210/pdb1gki/pdb
PDB entry 1gki coloured by chain, front view.
PDB entry 1gki coloured by chain, side view.
PDB entry 1gki coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase.
Gomis-Rüth FX, Moncalián G, Pérez-Luque R, González A, Cabezón E, de la Cruz F, Coll M
Nature 409 637-41 (2001)
PMID: 11214325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV secretion system coupling protein TraD DNA-binding domain-containing protein Chains: A, B, D, E, F, G
Molecule details ›
Chains: A, B, D, E, F, G
Length: 437 amino acids
Theoretical weight: 48.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04230 (Residues: 71-507; Coverage: 86%)
Gene name: trwB
Sequence domains: Type IV secretion-system coupling protein DNA-binding domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 5 x MG
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 151.1Å b: 151.1Å c: 251.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.232
Expression system: Escherichia coli

Quick links

Citations

73 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
72 more

11 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
10 more