› 1gis

X-ray diffraction
1.7Å resolution

A TRICHOSANTHIN(TCS) MUTANT(E85Q) COMPLEX STRUCTURE WITH 2'-DEOXY-ADENOSIN-5'-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1gis/pdb
PDB entry 1gis coloured by chain, front view.
PDB entry 1gis coloured by chain, side view.
PDB entry 1gis coloured by chain, top view.
Source organism: Trichosanthes kirilowii
Primary publication:
Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants.
Guo Q, Zhou W, Too HM, Li J, Liu Y, Bartlam M, Dong Y, Wong KB, Shaw PC, Rao Z
Protein Eng 16 391-6 (2003)
PMID: 12874371

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein alpha-trichosanthin Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 27.3 KDa
Source organism: Trichosanthes kirilowii
Expression system: Escherichia coli
UniProt:
  • Canonical: P09989 (Residues: 23-270; Coverage: 93%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DA 1 x DA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 38Å b: 75.9Å c: 78.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.234
Expression system: Escherichia coli

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Citations

2 review citations

Recent advances in trichosanthin, a ribosome-inactivating protein with multiple pharmacological properties.
Shaw et al. (2005)
1 more

2 mentions without citation

Structural and Functional Investigation and Pharmacological Mechanism of Trichosanthin, a Type 1 Ribosome-Inactivating Protein.
Shi et al. (2018)
1 more