› 1gg1

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE ANALYSIS OF DAHP SYNTHASE IN COMPLEX WITH MN2+ AND 2-PHOSPHOGLYCOLATE

Released:
DOI: 10.2210/pdb1gg1/pdb
PDB entry 1gg1 coloured by chain, front view.
PDB entry 1gg1 coloured by chain, side view.
PDB entry 1gg1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: comparison of the Mn(2+)*2-phosphoglycolate and the Pb(2+)*2-phosphoenolpyruvate complexes and implications for catalysis.
Wagner T, Shumilin IA, Bauerle R, Kretsinger RH
J Mol Biol 301 389-99 (2000)
PMID: 10926516

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 350 amino acids
Theoretical weight: 38.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB91 (Residues: 1-350; Coverage: 100%)
Gene names: JW0737, aroG, b0754
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand PGA 4 x PGA
Ligand MN 4 x MN
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 210.357Å b: 53.188Å c: 149.392Å
α: 90° β: 116.09° γ: 90°
R-values:
R R work R free
0.211 0.209 0.237
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Metabolic engineering of Escherichia coli for the production of phenylpyruvate derivatives.
Liu et al. (2015)
27 other articles

6 mentions without citation

Protein-protein docking with a reduced protein model accounting for side-chain flexibility.
Zacharias M. (2003)
5 more