› 1g8x

X-ray diffraction
2.8Å resolution

STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR

Released:
DOI: 10.2210/pdb1g8x/pdb
PDB entry 1g8x coloured by chain, front view.
PDB entry 1g8x coloured by chain, side view.
PDB entry 1g8x coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Structure of a genetically engineered molecular motor.
Kliche W, Fujita-Becker S, Kollmar M, Manstein DJ, Kull FJ
EMBO J 20 40-6 (2001)
PMID: 11226153

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin A; Myosin-2 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 1010 amino acids
Theoretical weight: 115.21 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 1-762, 763-764; Coverage: 36%)
  • Canonical: P05095 (Residues: 266-502; Coverage: 28%)
Gene names: DDB_G0268632, DDB_G0286355, abpA, actnA, mhcA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P21212
Unit cell:
a: 135.423Å b: 155.42Å c: 143.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.29
Expression system: Dictyostelium discoideum

Quick links

Citations

5 review citations

Dictyostelium discoideum--a promising expression system for the production of eukaryotic proteins.
Arya et al. (2008)
4 more

12 mentions without citation

Poorly understood aspects of striated muscle contraction.
Månsson et al. (2015)
11 more