› 1g0i

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE

Released:
DOI: 10.2210/pdb1g0i/pdb
PDB entry 1g0i coloured by chain, front view.
PDB entry 1g0i coloured by chain, side view.
PDB entry 1g0i coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure and catalytic mechanism of the MJ0109 gene product: a bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities.
Johnson KA, Chen L, Yang H, Roberts MF, Stec B
Biochemistry 40 618-30 (2001)
PMID: 11170378

Function and Biology Details

Reactions catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase/inositol-1-monophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.61 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57573 (Residues: 1-252; Coverage: 100%)
Gene names: MJ0109, suhB
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 6 x MN
Ligand PO4 2 x PO4
Ligand INS 2 x INS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 68.02Å b: 78.45Å c: 130.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.251 0.339
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Emerging themes in manganese transport, biochemistry and pathogenesis in bacteria.
Kehres et al. (2003)
3 more

1 mention without citation

A structural basis for lithium and substrate binding of an inositide phosphatase.
Dollins et al. (2021)