› 1flr

X-ray diffraction
1.85Å resolution

4-4-20 FAB FRAGMENT

Released:
DOI: 10.2210/pdb1flr/pdb
PDB entry 1flr coloured by chain, front view.
PDB entry 1flr coloured by chain, side view.
PDB entry 1flr coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
1.85 A structure of anti-fluorescein 4-4-20 Fab.
Whitlow M, Howard AJ, Wood JF, Voss EW, Hardman KD
Protein Eng 8 749-61 (1995)
PMID: 8637844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208647 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.17 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.97 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-100; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand FLU 1 x FLU
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 42.75Å b: 43.87Å c: 58.17Å
α: 95.15° β: 86.85° γ: 98.01°
R-values:
R R work R free
0.188 not available not available

Quick links

Citations

3 review citations

Lipocalins as a scaffold.
Skerra A. (2000)
2 more

22 mentions without citation

Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.
Boder et al. (2000)
21 more