› 1fjm

X-ray diffraction
2.1Å resolution

Protein serine/threonine phosphatase-1 (alpha isoform, type 1) complexed with microcystin-LR toxin

Released:
DOI: 10.2210/pdb1fjm/pdb
PDB entry 1fjm coloured by chain, front view.
PDB entry 1fjm coloured by chain, side view.
PDB entry 1fjm coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1.
Goldberg J, Huang HB, Kwon YG, Greengard P, Nairn AC, Kuriyan J
Nature 376 745-53 (1995)
PMID: 7651533

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158568 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.56 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62139 (Residues: 1-330; Coverage: 100%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
microcystin LR Chains: M, N
Molecule details ›
Chains: M, N
Length: 7 amino acids
Theoretical weight: 1.01 KDa
Source organism: Microcystis aeruginosa

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand BME 2 x BME
3 modified residues:
Ligand DAL 2 x DAL
Ligand ACB 2 x ACB
Ligand FGA 2 x FGA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.74Å b: 77.11Å c: 130.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available

Quick links

Citations

72 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
71 more

25 mentions without citation

The extended PP1 toolkit: designed to create specificity.
Bollen et al. (2010)
24 more