› 1fcv

X-ray diffraction
2.65Å resolution

CRYSTAL STRUCTURE OF BEE VENOM HYALURONIDASE IN COMPLEX WITH HYALURONIC ACID TETRAMER

Released:
DOI: 10.2210/pdb1fcv/pdb
PDB entry 1fcv coloured by chain, front view.
PDB entry 1fcv coloured by chain, side view.
PDB entry 1fcv coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Crystal structure of hyaluronidase, a major allergen of bee venom.
Marković-Housley Z, Miglierini G, Soldatova L, Rizkallah PJ, Müller U, Schirmer T
Structure 8 1025-35 (2000)
PMID: 11080624

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hyaluronidase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.9 KDa
Source organism: Apis mellifera
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q08169 (Residues: 33-382; Coverage: 99%)
Sequence domains: Hyaluronidase
Structure domains: Aldolase class I

Ligands and Environments

Carbohydrate polymer : NEW Components: GCU, NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P3221
Unit cell:
a: 71.012Å b: 71.012Å c: 151.326Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.163 0.252
Expression system: Trichoplusia ni

Quick links

Citations

34 review citations

The magic glue hyaluronan and its eraser hyaluronidase: a biological overview.
Girish et al. (2007)
33 more

32 mentions without citation

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
31 more