› 1fc5

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN

Released:
DOI: 10.2210/pdb1fc5/pdb
PDB entry 1fc5 coloured by chain, front view.
PDB entry 1fc5 coloured by chain, side view.
PDB entry 1fc5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of Escherichia coli MoeA, a protein from the molybdopterin synthesis pathway.
Schrag JD, Huang W, Sivaraman J, Smith C, Plamondon J, Larocque R, Matte A, Cygler M
J Mol Biol 310 419-31 (2001)
PMID: 11428898

Function and Biology Details

Reaction catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdopterin molybdenumtransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 44.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12281 (Residues: 1-411; Coverage: 100%)
Gene names: JW0811, b0827, bisB, chlE, moeA, narE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 76.64Å b: 101.85Å c: 103.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.253 0.297
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
3 more

5 mentions without citation

Contribution of structural genomics to understanding the biology of Escherichia coli.
Matte et al. (2003)
4 more