› 1f65

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF OXY SPERM WHALE MYOGLOBIN MUTANT Y(B10)Q(E7)R(E10)

Released:
DOI: 10.2210/pdb1f65/pdb
PDB entry 1f65 coloured by chain, front view.
PDB entry 1f65 coloured by chain, side view.
PDB entry 1f65 coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Structural dynamics of ligand diffusion in the protein matrix: A study on a new myoglobin mutant Y(B10) Q(E7) R(E10).
Brunori M, Cutruzzolà F, Savino C, Travaglini-Allocatelli C, Vallone B, Gibson QH
Biophys J 76 1259-69 (1999)
PMID: 10049310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.46 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand HEM 1 x HEM
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6
Unit cell:
a: 90.33Å b: 90.33Å c: 45.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.18 0.23
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Modeling heme proteins using atomistic simulations.
Bikiel et al. (2006)
6 more

1 mention without citation

Water stabilizes an alternate turn conformation in horse heart myoglobin.
Bronstein et al. (2023)