› 1f4s

Solution NMR

STRUCTURE OF TRANSCRIPTIONAL FACTOR ALCR IN COMPLEX WITH A TARGET DNA

Released:
DOI: 10.2210/pdb1f4s/pdb
PDB entry 1f4s coloured by chain, ensemble of 10 models, front view.
PDB entry 1f4s coloured by chain, ensemble of 10 models, side view.
PDB entry 1f4s coloured by chain, ensemble of 10 models, top view.
Source organism: Aspergillus nidulans
Primary publication:
The solution structure of an AlcR-DNA complex sheds light onto the unique tight and monomeric DNA binding of a Zn(2)Cys(6) protein.
Cahuzac B, Cerdan R, Felenbok B, Guittet E
Structure 9 827-36 (2001)
PMID: 11566132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113789 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Regulatory protein alcR Chain: P
Molecule details ›
Chain: P
Length: 65 amino acids
Theoretical weight: 7.58 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P21228 (Residues: 1-60; Coverage: 7%)
Gene names: AN8978, alcR
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains: Zn(2)-C6 fungal-type DNA-binding domain
DNA (5'-D(P*CP*GP*TP*GP*CP*GP*GP*AP*TP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided
DNA (5'-D(P*GP*AP*TP*CP*CP*GP*CP*AP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

A fungal family of transcriptional regulators: the zinc cluster proteins.
MacPherson et al. (2006)
1 more

1 mention without citation

Knowledge-based prediction of DNA hydration using hydrated dinucleotides as building blocks.
Biedermannová et al. (2022)