› 1ex2

X-ray diffraction
1.85Å resolution

CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN

Released:
DOI: 10.2210/pdb1ex2/pdb
PDB entry 1ex2 coloured by chain, front view.
PDB entry 1ex2 coloured by chain, side view.
PDB entry 1ex2 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Functional implications from crystal structures of the conserved Bacillus subtilis protein Maf with and without dUTP.
Minasov G, Teplova M, Stewart GC, Koonin EV, Anderson WF, Egli M
Proc Natl Acad Sci U S A 97 6328-33 (2000)
PMID: 10841541

Function and Biology Details

Reaction catalysed: 
A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
dTTP/UTP pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.32 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02169 (Residues: 1-189; Coverage: 100%)
Gene names: BSU28050, maf
Sequence domains: Maf-like protein
Structure domains: Maf protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 62.66Å b: 86.01Å c: 93.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.223
Expression system: Escherichia coli

Quick links

Citations

5 review citations

A tour of structural genomics.
Brenner SE. (2001)
4 more

4 mentions without citation

Functional implications from crystal structures of the conserved Bacillus subtilis protein Maf with and without dUTP.
Minasov et al. (2000)
3 more