› 1evx

X-ray diffraction
2Å resolution

APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI

Released:
DOI: 10.2210/pdb1evx/pdb
PDB entry 1evx coloured by chain, front view.
PDB entry 1evx coloured by chain, side view.
PDB entry 1evx coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.
Galburt EA, Chadsey MS, Jurica MS, Chevalier BS, Erho D, Tang W, Monnat RJ, Stoddard BL
J Mol Biol 300 877-87 (2000)
PMID: 10891275

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intron-encoded endonuclease I-PpoI Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 17.68 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94702 (Residues: 2-163; Coverage: 99%)
Sequence domains: Zinc-binding loop region of homing endonuclease
Structure domains: Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 52.8Å b: 52.8Å c: 278.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.229
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
1 more

2 mentions without citation

A protein-DNA docking benchmark.
van Dijk et al. (2008)
1 more