› 1etq

X-ray diffraction
2.8Å resolution

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71Y

Released:
DOI: 10.2210/pdb1etq/pdb
PDB entry 1etq coloured by chain, front view.
PDB entry 1etq coloured by chain, side view.
PDB entry 1etq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of the transcriptional activation region on Fis: crystal structures of six Fis mutants with different activation properties.
Cheng YS, Yang WZ, Johnson RC, Yuan HS
J Mol Biol 302 1139-51 (2000)
PMID: 11183780

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein Fis Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6R3 (Residues: 1-98; Coverage: 100%)
Gene names: JW3229, b3261, fis
Sequence domains: Bacterial regulatory protein, Fis family
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 50.21Å b: 57.97Å c: 135.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.277
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Post-translational modification of nucleoid-associated proteins: an extra layer of functional modulation in bacteria?
Dilweg et al. (2018)
2 more

1 mention without citation

Crystal structure of the nucleoid-associated protein Fis (PA4853) from Pseudomonas aeruginosa.
Zhou et al. (2020)