› 1epv

X-ray diffraction
2.2Å resolution

ALANINE RACEMASE WITH BOUND INHIBITOR DERIVED FROM D-CYCLOSERINE

Released:
DOI: 10.2210/pdb1epv/pdb
PDB entry 1epv coloured by chain, front view.
PDB entry 1epv coloured by chain, side view.
PDB entry 1epv coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
A side reaction of alanine racemase: transamination of cycloserine.
Fenn TD, Stamper GF, Morollo AA, Ringe D
Biochemistry 42 5775-83 (2003)
PMID: 12741835

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 43.56 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10724 (Residues: 2-388; Coverage: 100%)
Gene names: alr, dal
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DCS 2 x DCS
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 99.157Å b: 90.243Å c: 85.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.226
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Drugs, their targets and the nature and number of drug targets.
Imming et al. (2006)
7 more

3 mentions without citation

Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.
Lowther et al. (2010)
2 more