› 1eps

X-ray diffraction
3Å resolution

STRUCTURE AND TOPOLOGICAL SYMMETRY OF THE GLYPHOSPHATE 5-ENOL-PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE: A DISTINCTIVE PROTEIN FOLD

Released:
DOI: 10.2210/pdb1eps/pdb
PDB entry 1eps coloured by chain, front view.
PDB entry 1eps coloured by chain, side view.
PDB entry 1eps coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and topological symmetry of the glyphosate target 5-enolpyruvylshikimate-3-phosphate synthase: a distinctive protein fold.
Stallings WC, Abdel-Meguid SS, Lim LW, Shieh HS, Dayringer HE, Leimgruber NK, Stegeman RA, Anderson KS, Sikorski JA, Padgette SR, Kishore GM
Proc Natl Acad Sci U S A 88 5046-50 (1991)
PMID: 11607190

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.21 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A6D3 (Residues: 1-427; Coverage: 100%)
Gene names: JW0891, aroA, b0908
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 92.1Å b: 83.2Å c: 72.1Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

8 review citations

Evaluation of protein safety in the context of agricultural biotechnology.
Delaney et al. (2008)
7 more

5 mentions without citation

Molecular basis of glyphosate resistance-different approaches through protein engineering.
Pollegioni et al. (2011)
4 more