› 1ep8

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A MUTATED THIOREDOXIN, D30A, FROM CHLAMYDOMONAS REINHARDTII

Released:
DOI: 10.2210/pdb1ep8/pdb
PDB entry 1ep8 coloured by chain, front view.
PDB entry 1ep8 coloured by chain, side view.
PDB entry 1ep8 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Crystal structure of the wild-type and D30A mutant thioredoxin h of Chlamydomonas reinhardtii and implications for the catalytic mechanism.
Menchise V, Corbier C, Didierjean C, Saviano M, Benedetti E, Jacquot JP, Aubry A
Biochem J 359 65-75 (2001)
PMID: 11563970

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin H-type Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 11.68 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P80028 (Residues: 2-113; Coverage: 99%)
Gene name: TRXH
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P3121
Unit cell:
a: 49.692Å b: 49.692Å c: 145.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.22
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural and evolutionary aspects of thioredoxin reductases in photosynthetic organisms.
Jacquot et al. (2009)
5 more