› 1eji

X-ray diffraction
2.9Å resolution

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)

Released:
DOI: 10.2210/pdb1eji/pdb
PDB entry 1eji coloured by chain, front view.
PDB entry 1eji coloured by chain, side view.
PDB entry 1eji coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers.
Szebenyi DM, Liu X, Kriksunov IA, Stover PJ, Thiel DJ
Biochemistry 39 13313-23 (2000)
PMID: 11063567

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase, cytosolic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 478 amino acids
Theoretical weight: 53.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P50431 (Residues: 1-478; Coverage: 100%)
Gene names: Shmt, Shmt1
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand THF 3 x THF
1 bound ligand:
Ligand PLG 4 x PLG
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P43212
Unit cell:
a: 142.52Å b: 142.52Å c: 270.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.271 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Serine hydroxymethyltransferase revisited.
Schirch et al. (2005)
3 more

8 mentions without citation

A pyrazolopyran derivative preferentially inhibits the activity of human cytosolic serine hydroxymethyltransferase and induces cell death in lung cancer cells.
Marani et al. (2016)
7 more