› 1ej1

X-ray diffraction
2.2Å resolution

COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP

Released:
DOI: 10.2210/pdb1ej1/pdb
PDB entry 1ej1 coloured by chain, front view.
PDB entry 1ej1 coloured by chain, side view.
PDB entry 1ej1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP.
Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK
Cell 89 951-61 (1997)
PMID: 9200613

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63073 (Residues: 28-217; Coverage: 88%)
Gene name: Eif4e
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand M7G 2 x M7G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 69.6Å b: 75.4Å c: 76.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.235
Expression system: Escherichia coli

Quick links

Citations

97 review citations

Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
Filipowicz et al. (2008)
96 more

14 mentions without citation

Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex.
Decroly et al. (2011)
13 more