› 1ecr

X-ray diffraction
2.7Å resolution

ESCHERICHIA COLI REPLICATION TERMINATOR PROTEIN (TUS) COMPLEXED WITH DNA

Released:
DOI: 10.2210/pdb1ecr/pdb
PDB entry 1ecr coloured by chain, front view.
PDB entry 1ecr coloured by chain, side view.
PDB entry 1ecr coloured by chain, top view.
Source organism: Escherichia coli B
Primary publication:
Structure of a replication-terminator protein complexed with DNA.
Kamada K, Horiuchi T, Ohsumi K, Shimamoto N, Morikawa K
Nature 383 598-603 (1996)
PMID: 8857533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113726 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication terminus site-binding protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.84 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
  • Canonical: P16525 (Residues: 1-309; Coverage: 100%)
Gene names: JW1602, b1610, tau, tus
Sequence domains: DNA replication terminus site-binding protein (Ter protein)
Structure domains:
DNA (5'-D(*TP*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*CP*T)-3 Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Escherichia coli B
Expression system: Not provided
DNA (5'-D(*TP*AP*GP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3 Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Escherichia coli B
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P41212
Unit cell:
a: 68.15Å b: 68.15Å c: 230.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.314
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Replication and control of circular bacterial plasmids.
del Solar et al. (1998)
19 more

13 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
12 more