› 1ec6

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN

Released:
DOI: 10.2210/pdb1ec6/pdb
PDB entry 1ec6 coloured by chain, front view.
PDB entry 1ec6 coloured by chain, side view.
PDB entry 1ec6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome.
Lewis HA, Musunuru K, Jensen KB, Edo C, Chen H, Darnell RB, Burley SK
Cell 100 323-32 (2000)
PMID: 10676814

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194021 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Nova-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 9.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNW9 (Residues: 406-491; Coverage: 18%)
Gene names: ANOVA, NOVA2, NOVA3
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
20-MER RNA HAIRPIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 47.85Å b: 51.21Å c: 128.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.208 0.291
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

57 review citations

RNA-binding proteins in human genetic disease.
Lukong et al. (2008)
56 more

29 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
28 more