› 1eao

X-ray diffraction
1.4Å resolution

THE RUNX1 Runt domain at 1.4A resolution: a structural switch and specifically bound chloride ions modulate DNA binding

Released:
DOI: 10.2210/pdb1eao/pdb
PDB entry 1eao coloured by chain, front view.
PDB entry 1eao coloured by chain, side view.
PDB entry 1eao coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding.
Bäckström S, Wolf-Watz M, Grundström C, Härd T, Grundström T, Sauer UH
J Mol Biol 322 259-72 (2002)
PMID: 12217689

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Runt-related transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.52 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03347 (Residues: 46-185; Coverage: 31%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand BR 4 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 91.066Å b: 46.173Å c: 62.904Å
α: 90° β: 91.94° γ: 90°
R-values:
R R work R free
0.143 0.141 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Runx2: a master organizer of gene transcription in developing and maturing osteoblasts.
Schroeder et al. (2005)
6 more

3 mentions without citation

Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL.
Della Gatta et al. (2012)
2 more