› 1e8o

X-ray diffraction
3.2Å resolution

Core of the Alu domain of the mammalian SRP

Released:
DOI: 10.2210/pdb1e8o/pdb
PDB entry 1e8o coloured by chain, front view.
PDB entry 1e8o coloured by chain, side view.
PDB entry 1e8o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and assembly of the Alu domain of the mammalian signal recognition particle.
Weichenrieder O, Wild K, Strub K, Cusack S
Nature 408 167-73 (2000)
PMID: 11089964

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153358 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 9 kDa protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 85 amino acids
Theoretical weight: 10 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49458 (Residues: 2-86; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
Signal recognition particle 14 kDa protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 12.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37108 (Residues: 2-107; Coverage: 78%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
7SL RNA Chain: E
Molecule details ›
Chain: E
Length: 50 nucleotides
Theoretical weight: 16.28 KDa

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand GDP 1 x GDP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 57.448Å b: 186.621Å c: 189.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.291
Expression system: Escherichia coli

Quick links

Citations

27 review citations

The signal recognition particle.
Keenan et al. (2001)
26 more

19 mentions without citation

Structure, function and evolution of the signal recognition particle.
Nagai et al. (2003)
18 more