› 1e7d

X-ray diffraction
2.8Å resolution

Endonuclease VII (ENDOVII) Ffrom Phage T4

Released:
DOI: 10.2210/pdb1e7d/pdb
PDB entry 1e7d coloured by chain, front view.
PDB entry 1e7d coloured by chain, side view.
PDB entry 1e7d coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Conformational flexibility in T4 endonuclease VII revealed by crystallography: implications for substrate binding and cleavage.
Raaijmakers H, Törö I, Birkenbihl R, Kemper B, Suck D
J Mol Biol 308 311-23 (2001)
PMID: 11327769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombination endonuclease VII Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.17 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13340 (Residues: 1-157; Coverage: 100%)
Gene name: 49
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P21
Unit cell:
a: 60.9Å b: 37.4Å c: 74Å
α: 90° β: 108.2° γ: 90°
R-values:
R R work R free
0.245 0.245 0.315
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Holliday junction resolvases.
Wyatt et al. (2014)
2 more

6 mentions without citation

Bacteriophage T4 genome.
Miller et al. (2003)
5 more