› 1e4e

X-ray diffraction
2.5Å resolution

D-alanyl-D-lacate ligase

Released:
DOI: 10.2210/pdb1e4e/pdb
PDB entry 1e4e coloured by chain, front view.
PDB entry 1e4e coloured by chain, side view.
PDB entry 1e4e coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).
Roper DI, Huyton T, Vagin A, Dodson G
Proc Natl Acad Sci U S A 97 8921-5 (2000)
PMID: 10908650

Function and Biology Details

Reaction catalysed: 
D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150334 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vancomycin/teicoplanin A-type resistance protein VanA Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.58 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: P25051 (Residues: 1-343; Coverage: 100%)
Gene name: vanA
Sequence domains:
Structure domains:
Vancomycin/teicoplanin A-type resistance protein VanA Chain: B
Molecule details ›
Chain: B
Length: 343 amino acids
Theoretical weight: 37.62 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: P25051 (Residues: 1-343; Coverage: 100%)
Gene name: vanA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADP 2 x ADP
Ligand PHY 2 x PHY
Ligand MG 4 x MG
Ligand SO4 3 x SO4
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 123.204Å b: 225.359Å c: 72.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.257
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
6 more

10 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
9 more