› 1e2b

Solution NMR

NMR STRUCTURE OF THE C10S MUTANT OF ENZYME IIB CELLOBIOSE OF THE PHOSPHOENOL-PYRUVATE DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI, 17 STRUCTURES

Released:
DOI: 10.2210/pdb1e2b/pdb
PDB entry 1e2b coloured by chain, ensemble of 17 models, front view.
PDB entry 1e2b coloured by chain, ensemble of 17 models, side view.
PDB entry 1e2b coloured by chain, ensemble of 17 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.
Ab E, Schuurman-Wolters G, Reizer J, Saier MH, Dijkstra K, Scheek RM, Robillard GT
Protein Sci 6 304-14 (1997)
PMID: 9041631

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system N,N'-diacetylchitobiose-specific EIIB component Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P69795 (Residues: 1-106; Coverage: 100%)
Gene names: JW1727, b1738, celA, chbB
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS, SIMULATED ANNEALING
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links

Citations

2 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
1 more

2 mentions without citation

Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
Jung et al. (2010)
1 more