Function and Biology Details
Reactions catalysed:
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
Molecule details ›
Chains: A, B
Length: 512 amino acids
Theoretical weight: 58.47 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases
Length: 512 amino acids
Theoretical weight: 58.47 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
- Canonical:
P49235 (Residues: 55-566; Coverage: 91%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-3
Spacegroup:
P21
Expression system: Escherichia coli
