Function and Biology Details
Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:
Structure analysis Details
Assembly composition:
hetero nonamer (preferred)
Assembly name:
Thrombininhibitor and Thrombin heavy chain (preferred)
PDBe Complex ID:
PDB-CPX-133160 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain
Molecule details ›
Chains: A, B, C
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Thrombin light chain
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P00734 (Residues: 328-363; Coverage: 6%)
Sequence domains: Thrombin light chain
Thrombin heavy chain
Molecule details ›
Chains: D, E, F
Length: 259 amino acids
Theoretical weight: 29.79 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Length: 259 amino acids
Theoretical weight: 29.79 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Thrombininhibitor
Molecule details ›
Chains: I, J, K
Length: 57 amino acids
Theoretical weight: 6.26 KDa
Source organism: Haemadipsa sylvestris
Expression system: Escherichia coli DH5[alpha]
UniProt:
Structure domains: Thrombin Inhibitor (Hirudin), subunit I
Length: 57 amino acids
Theoretical weight: 6.26 KDa
Source organism: Haemadipsa sylvestris
Expression system: Escherichia coli DH5[alpha]
UniProt:
- Canonical: Q25163 (Residues: 21-77; Coverage: 100%)
Structure domains: Thrombin Inhibitor (Hirudin), subunit I
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU RUH2R
Spacegroup:
P2
Expression system: Escherichia coli DH5[alpha]
