› 1dp2

X-ray diffraction
2.01Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RHODANESE AND LIPOATE

Released:
DOI: 10.2210/pdb1dp2/pdb
PDB entry 1dp2 coloured by chain, front view.
PDB entry 1dp2 coloured by chain, side view.
PDB entry 1dp2 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Specific interaction of lipoate at the active site of rhodanese.
Cianci M, Gliubich F, Zanotti G, Berni R
Biochim Biophys Acta 1481 103-8 (2000)
PMID: 11004580

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 32.98 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00586 (Residues: 2-294; Coverage: 99%)
Gene name: TST
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

1 bound ligand:
Ligand LPB 1 x LPB
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: C2
Unit cell:
a: 154.36Å b: 49.31Å c: 41.68Å
α: 90° β: 99.6° γ: 90°
R-values:
R R work R free
0.185 0.17 0.23

Quick links

Citations

3 review citations

Enzymatic activation of sulfur for incorporation into biomolecules in prokaryotes.
Kessler D. (2006)
2 more

4 mentions without citation

Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase.
Yadav et al. (2013)
3 more