› 1dj3

X-ray diffraction
3Å resolution

STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA

Released:
DOI: 10.2210/pdb1dj3/pdb
PDB entry 1dj3 coloured by chain, front view.
PDB entry 1dj3 coloured by chain, side view.
PDB entry 1dj3 coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana.
Prade L, Cowan-Jacob SW, Chemla P, Potter S, Ward E, Fonne-Pfister R
J Mol Biol 296 569-77 (2000)
PMID: 10669609

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 47.79 KDa
Source organism: Triticum aestivum
Expression system: Escherichia coli
UniProt:
  • Canonical: O24396 (Residues: 35-476; Coverage: 93%)
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P41212
Unit cell:
a: 151.44Å b: 151.44Å c: 91.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.303
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Purine and pyrimidine nucleotide metabolism in higher plants.
Stasolla et al. (2003)
12 other articles

1 mention without citation

sc-PDB: a 3D-database of ligandable binding sites--10 years on.
Desaphy et al. (2015)