› 1dj0

X-ray diffraction
1.5Å resolution

THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1dj0/pdb
PDB entry 1dj0 coloured by chain, front view.
PDB entry 1dj0 coloured by chain, side view.
PDB entry 1dj0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I.
Foster PG, Huang L, Santi DV, Stroud RM
Nat Struct Biol 7 23-7 (2000)
PMID: 10625422

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07649 (Residues: 7-270; Coverage: 98%)
Gene names: JW2315, asuC, b2318, hisT, leuK, truA
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 68.08Å b: 37.4Å c: 103.48Å
α: 90° β: 97.68° γ: 90°
R-values:
R R work R free
0.162 0.167 0.221
Expression system: Escherichia coli

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Citations

16 review citations

The role of m6A, m5C and Ψ RNA modifications in cancer: Novel therapeutic opportunities.
Nombela et al. (2021)
15 more

8 mentions without citation

Prediction of catalytic residues using Support Vector Machine with selected protein sequence and structural properties.
Petrova et al. (2006)
7 more