› 1dcn

X-ray diffraction
2.3Å resolution

INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE

Released:
DOI: 10.2210/pdb1dcn/pdb
PDB entry 1dcn coloured by chain, front view.
PDB entry 1dcn coloured by chain, side view.
PDB entry 1dcn coloured by chain, top view.
Source organism: Anas platyrhynchos
Primary publication:
Crystal structure of an inactive duck delta II crystallin mutant with bound argininosuccinate.
Vallée F, Turner MA, Lindley PL, Howell PL
Biochemistry 38 2425-34 (1999)
PMID: 10029536

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 49.5 KDa
Source organism: Anas platyrhynchos
Expression system: Escherichia coli
UniProt:
  • Canonical: P24058 (Residues: 19-465; Coverage: 96%)
Gene name: ASL2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AS1 1 x AS1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 94.58Å b: 99.59Å c: 107.14Å
α: 90° β: 101.68° γ: 90°
R-values:
R R work R free
0.229 0.229 0.29
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Argininosuccinate lyase deficiency.
Nagamani et al. (2012)
4 more