› 1d1m

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF CRO K56-[DGEVK]-F58W MUTANT

Released:
DOI: 10.2210/pdb1d1m/pdb
PDB entry 1d1m coloured by chain, front view.
PDB entry 1d1m coloured by chain, side view.
PDB entry 1d1m coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
The structural basis for enhanced stability and reduced DNA binding seen in engineered second-generation Cro monomers and dimers.
Rupert PB, Mollah AK, Mossing MC, Matthews BW
J Mol Biol 296 1079-90 (2000)
PMID: 10686105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.3 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 1-56, 57-60; Coverage: 91%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 36.03Å b: 35.66Å c: 108.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.298
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
5 other articles

1 mention without citation

Research progress on solutions to the sneak path issue in memristor crossbar arrays.
Shi et al. (2020)