› 1cvz

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE ANALYSIS OF PAPAIN WITH CLIK148(CATHEPSIN L SPECIFIC INHIBITOR)

Released:
DOI: 10.2210/pdb1cvz/pdb
PDB entry 1cvz coloured by chain, front view.
PDB entry 1cvz coloured by chain, side view.
PDB entry 1cvz coloured by chain, top view.
Source organism: Carica papaya
Primary publication:
Inhibition mechanism of cathepsin L-specific inhibitors based on the crystal structure of papain-CLIK148 complex.
Tsuge H, Nishimura T, Tada Y, Asao T, Turk D, Turk V, Katunuma N
Biochem Biophys Res Commun 266 411-6 (1999)
PMID: 10600517

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Papain Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.45 KDa
Source organism: Carica papaya
UniProt:
  • Canonical: P00784 (Residues: 134-345; Coverage: 65%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand C48 1 x C48
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 42.89Å b: 49.91Å c: 95.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.214

Quick links

Citations

10 review citations

Cysteine cathepsins: from structure, function and regulation to new frontiers.
Turk et al. (2012)
9 more

11 mentions without citation

SARS-CoV-2 Entry Inhibitors: Small Molecules and Peptides Targeting Virus or Host Cells.
Cannalire et al. (2020)
10 more