› 1cvj

X-ray diffraction
2.6Å resolution

X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA

Released:
DOI: 10.2210/pdb1cvj/pdb
PDB entry 1cvj coloured by chain, front view.
PDB entry 1cvj coloured by chain, side view.
PDB entry 1cvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of polyadenylate RNA by the poly(A)-binding protein.
Deo RC, Bonanno JB, Sonenberg N, Burley SK
Cell 98 835-45 (1999)
PMID: 10499800

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146002 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polyadenylate-binding protein 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 190 amino acids
Theoretical weight: 21.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11940 (Residues: 1-190; Coverage: 30%)
Gene names: PAB1, PABP, PABP1, PABPC1, PABPC2
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3' Chains: M, N, O, P, Q, R, S, T
Molecule details ›
Chains: M, N, O, P, Q, R, S, T
Length: 11 nucleotides
Theoretical weight: 3.58 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand A 8 x A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, CHESS BEAMLINE F1, NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 79.557Å b: 176.797Å c: 82.489Å
α: 90° β: 117.67° γ: 90°
R-values:
R R work R free
0.23 0.23 0.304
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

49 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
48 more

48 mentions without citation

Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.
Mangus et al. (2003)
47 more