› 1csp

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS MAJOR COLD SHOCK PROTEIN, CSPB: A UNIVERSAL NUCLEIC-ACID BINDING DOMAIN

Released:
DOI: 10.2210/pdb1csp/pdb
PDB entry 1csp coloured by chain, front view.
PDB entry 1csp coloured by chain, side view.
PDB entry 1csp coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein.
Schindelin H, Marahiel MA, Heinemann U
Nature 364 164-8 (1993)
PMID: 8321288

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cold shock protein CspB Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P32081 (Residues: 1-67; Coverage: 100%)
Gene names: BSU09100, cspA, cspB
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 58.94Å b: 58.94Å c: 46.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 not available not available
Expression system: Escherichia coli

Quick links

Citations

32 review citations

The TetR family of transcriptional repressors.
Ramos et al. (2005)
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61 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
60 more