› 1ckm

X-ray diffraction
2.5Å resolution

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP

Released:
DOI: 10.2210/pdb1ckm/pdb
PDB entry 1ckm coloured by chain, front view.
PDB entry 1ckm coloured by chain, side view.
PDB entry 1ckm coloured by chain, top view.
Source organism: Paramecium bursaria Chlorella virus 1
Primary publication:
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.
Håkansson K, Doherty AJ, Shuman S, Wigley DB
Cell 89 545-53 (1997)
PMID: 9160746

Function and Biology Details

Reaction catalysed: 
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.88 KDa
Source organism: Paramecium bursaria Chlorella virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84424 (Residues: 1-330; Coverage: 100%)
Gene name: A103R
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: C2221
Unit cell:
a: 93.332Å b: 214.931Å c: 105.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.299
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Viral and cellular mRNA capping: past and prospects.
Furuichi et al. (2000)
25 more

16 mentions without citation

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
15 more